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KMID : 0665220140270030400
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Korean Journal of Food and Nutrition
2014 Volume.27 No. 3 p.400 ~ p.405
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Secretion of Inflammatory Cytokines by Aloe vera Extract in HepG2 Cells
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Kim Il-Rang
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Abstract
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Recently, cases of Aloe vera induced-toxic hepatitis have been reported. However, the precise inflammatory effects of Aloe vera extract have not been clearly elucidated yet. In this study, the inflammatory effects and the mechanism of 70% ethanolic Aloe vera extract on liver were evaluated by in vitro assays using human hepatoma HepG2 cells. Cell viability was investigated using MTT assay at 0.001~100 μg/mL of Aloe vera extract. To evaluate inflammatory effect of the Aloe vera extract, the secretion of pro-inflammatory cytokine Interleukin 8 (IL-8) and Macrophage colony-stimulating factor (M-CSF) were detected. Aloe vera extract did not induce cell death at concentrations of 0.001~100 μg/mL. However, Aloe vera extract significantly increased the IL-8 secretion by 15.7~25.8% and the M-CSF secretion by 36.6~61.5% at the same concentrations. These results indicate that Aloe vera extract shows an inflammation-related mild hepatotoxicity than a severe toxicity such as cell death and this hepatitis is mediated by the secretion of inflammatory cytokine IL-8 and M-CSF.
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KEYWORD
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Aloe vera, HepG2, inflammation, hepatitis, cytokine
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